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Primers used for cloning are listed in Supplementary Table 3. Anti-HA antibody was obtained from Cell Signalling Technology (C29F4; 1:2000 dilution western blot).
Network Rail said it was investing £350m to improve signalling technology.
Both track and signalling technology (TVM-430 + KVB) are based on or identical to the standards used on the French LGV high-speed lines.
Phospho-p38MAPK (Thr180/Tyr182) and p38MAPK were purchased from Cell Signalling Technology, Inc. (Milan, Italy).
The membranes were incubated with an anti-mouse HRP-linked secondary antibody (1∶2000, Cell Signalling Technology) for 1 h.
and caspase 9 & cleaved caspase 9 (1∶200; 9509, Cell Signalling Technology, Danvers, MA) overnight at 4°C or for 2 h at room temperature.
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Several dilutions of P-CREB and CREB antibodies (Cell Signalling Technology, cat# 9191 and 9192, respectively) were tested to establish 1 100 as optimal.
Calyculin A was purchased from Cell Signalling Technology.
PD 98059 (no. 9900) was purchased from Cell Signalling Technology.
Caspase activation was assessed using rabbit anti-caspase 3 (Cell Signalling Technology), rabbit anti-cleaved caspase 3 (Cell Signalling Technology), rabbit anti-caspase 9 antibody (Cell Signalling Technology), and rabbit anti-cleaved caspase 9 (Cell Signalling Technology) antibodies.
Two caspase-3 antibodies were purchased from Cell Signalling Technology.
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