Due to the proportional statistical over-representation of signal transduction pathways in the zones closest to the centre, their known importance in cellular functions and their prominence as a drug target category, we explored the distribution of proteins having any signalling function, as well as functions related to regulation of signalling (Table 4).
NFAP aggravated the toxicity of 8-Br-cAMP indicating a synergistic interaction between the two compounds and an activating effect of NFAP in cAMP/Pka signalling (Table 2).
In terms of Biological Processes significant representation (p<0.05) was found for cell proliferation and differentiation, apoptosis and intracellular signalling (Table 2).
We also analysed known cellular pathways and found the pathways with the highest number of genes from the overlapping set included those involved in cell cycle, endoplasmic reticulum stress and Ras signalling (Table 8).
However, information on signaling functions of, or selection pressures on, colour appears only available for patches that look conspicuous to the human eye, which are thus assumed to be important in signalling (Table 1).
The current licensed targeted therapies all inhibit VEGF signalling (Table 1).
Interestingly, genes with reduced methylation were also involved in pathways such as ephrin B signalling (Table 2b).
This was correlated with a differential expression of many genes involved in hormone biosynthesis or signalling (Table 1).
From the category cell-cell signalling (Table 4C) two out of the five transcripts were selected for further qPCR analysis.
The positive impact of erlotinib, the optimal treatment against RB signalling (Table 4), was supported by simulations.
In A549, TGF-β modulated genes belong to (a) MAP kinase signalling (table 5), (b) tight junction (table 6), (c) adherans junction (table 7) (d) focal adhesion (table 8) (e) insulin signalling (table 9) (f) regulation of actin cytoskeleton (table 10) and (g) Wnt signalling (table 11).
