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In cell-line transcriptional assays with reporter constructs driven by target gene promoters, the levels of the activated Smad2/3 (P-Smad2/3) reflect signalling intensity (ligand activated receptors) and these are proportionate to the levels of reporter expression.
Figure 4B summarizes the predicted miRNAs having potential in modulating Wnt signalling intensity in R-NSCs.
Crosstalk at multiple levels allows for promotion or attenuation of signalling intensity and specificity.
For example, previous studies have found that environmental factors [ 23, 30] and variation in female reproductive state [ 25, 31] can be important sources of within-male variation in male signalling intensity.
Such sensitivity is likely conferred by the participation of different PRRs, PAMP-PRR interactions or the accumulative signalling intensity (i.e. threshold) of the group of PRRs involved in recognition.
The probability of a SC to switch to maturation is much lower when signalling intensity in the micro-environment is reduced, whereas in micro-environments having a significantly weak intercellular communication, an uncontrolled proliferation is the only possible outcome.
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Signal intensity.
Fig. 3 Signal intensity.
Note normal marrow signal intensity (M).
Note high signal intensity on T2w.
c Colloidal gold strip signal intensity.
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