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ODQ may also not be able to block cGMP signalling in all circumstances.
To confirm that reporter expression in the absence of ectopic Notch activity reflects endogenous Notch signalling, we repeated the previous experiment, but in this case quenching endogenous Notch signalling in all electroporated cells by co-electroporating pCAG-CSLDN (a plasmid driving constitutive expression of a dominant negative form of CSL).
However, because Rht is a negative regulator of GA signalling in all tissues, these alleles are also associated with negative pleiotropic effects, such as the requirement for planting closer to the surface and a slight reduction in grain size [ 15, 19].
Although transient transfection of BMPR2 is a rather artificial method to activate the BMP pathway, we have previously shown that it is a reliable way of achieving robust activation of BMP signalling in all CRC cell lines by circumventing the loss of BMPR2 seen in a proportion of CRCs and variations in expression levels of BMP ligands and inhibitors (Kodach et al, 2008).
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The cord holding her office badge had "Mission Baghdadd" printed on it, sending signals in all directions.
The brain is a complex collection of billions of neurons, like a massive telephone exchange sending signals in all directions.
"Basically, we didn't have a coach-to-quarterback operation, so we had to signal in all of the plays, which is unusual, but that's the way it was," Belichick said in Foxborough, Mass.
There are several from which to choose, including an omnidirectional antenna designed to increase the network signal in all directions and a directional antenna that can be positioned to extend the network's range in a specific area.
Similarly, if procurement and business initiatives are to succeed, the government must set market signals in all that it does and not just from the departments who are directly tasked with green policy making.
In total fourteen (88%) FSUs have a reduced signal in all or any endplate.
Vibratome tissue section showed uniform GUS positive signals in all tissues and cells (Fig. 4b).
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