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We propose a specific EpoR-activated interface and suggest its use for structural and signaling studies.
Classic developmental model systems and transgenic animals are instructive but pose special challenges for investigators conducting signaling studies and biochemical assays in tissues.
Signaling studies confirmed that R100W and R100E hNGF mutants display super imposable properties.
Co-localization and signaling studies were predominantly carried out with keratinocytes from murine oral epithelium (MK).
Despite the growing understanding of PDGF signaling, studies of PDGF function have met two obstacles.
The recombinant hNGF and hproNGFR100 proteins were used for cell signaling studies in different cell lines.
TNF-α is a commonly used cytokine for redox signaling studies [59].
Co-localization and signaling studies were carried out with keratinocytes from murine oral epithelium (MK).
We have been among the pioneers in the field, with a primary focus on signaling studies [10] [13].
Our broader goal was to determine the suitability of ICW as a technique that may be substituted for WB in signaling studies, particularly for high-throughput experimental protocols.
For signaling studies, cells were washed and incubated in serum-free media (RPMI/0.1%BSA) devoid of all cytokines and incubated at 37°C for 4 hours.
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