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The complete experimental design called for each signal variant to be used across four behavioral tasks presented in the following order: control, timing+motor, timing, and motor.
We measured the area, morphometric characteristics, and width of identifiable signal variant regions on each image and compared these measurements with the histopathologic findings.
Performing this comparison revealed that an association P = 10−5 was sufficiently strict for the avoidance of false-positive results in the discovery of secondary signals within a region after adjusting for the top signal variant.
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Fisher's exact test reveals that the number of exon 36.1 splice signal variants found among the undetected alleles of the one-allele cohort (5/28) is significantly greater (P < 10−6) than among the ABCA4 alleles of controls (0/600).
Features such as the presence of multiple cleavage sites, distribution of poly(A) signal variants and nucleotide composition of flanking regions were reported to be similar in human and mouse [ 4].
In this section, we describe signaling variants for LTE systems.
Quantitative analysis indicated that cells expressing WT p130Cas have increased protrusion velocities relative to Vector cells and to all three p130Cas signaling variants (Fig. 3B).
As was observed for cell migration rate, the additive effect of the 15F and mPR signaling variants indicates that sustained FA disassembly is regulated by distinct p130Cas signaling functions of the SD and SBD.
MN survival is proportional to the amount of muscle in developing limbs in embryos with attenuated expression levels of Met-signalling variants.
Thus although switching signalling cascades activated by Met mutants leads to comparable efficacy to expand the Pea3 expression domain in the context of two functional copies of Pea3, the signalling routes employed by the Met signalling variants are differentially sensitive to Pea3 gene dosage reduction: the Met2S route is sensitive, whereas the Met2P is not.
To test the regulatory potential of this SNP, we further performed an allele-specific luciferase reporter assay and observed significantly lower signal in variant allele A than reference allele G (P = 1.13E−05 in dihydrotestosterone (DHT -treated cell line anDHT -treated03 in cell line without DHT treandent) (Fig. 4c).
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