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HER1 and HER2 antibodies were identified and conditions optimized for the highest signal to background detection of HER1-HER2 heterodimers.
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Analytical performance of the nebulization systems was characterized by determination of the signal to background ratio (SBR), the limits of detection (LODs), and the precision (RSDs).
It offers a 67-fold signal to background ratio, and more than 25-fold discriminating ability for mismatch detection is achieved.
Finally, detection and reconstruction of the weak periodic signal are achieved from the output SBNR (signal to background noise ratio).
Mismatches lowered tumor signals to background.
A high-fluorescence background associated with the current TaqMan probe sequence contributes to the relatively low detection sensitivity and signal-to-background ratio.
Compared to single-labeled FAM-MB based telomerase activity assay, the label-free AIE-MB induced method could perform the sensitive detection with high signal-to-background ratio.
This method allows bursts to be detected when the signal-to-background ratio is much smaller than 50%, and we demonstrate, with simulated data, detection of bursts from a 10% signal component contaminated by a uniform random background.
We discuss each detection approach in terms of signal-to-background (S/B) ratio, ease of use, and adaptability to high-throughput format.
For detection, the PSF reconstruction parameters should optimize the signal-to-background ratio while also accounting for image noise, which was not studied here.
By simple direct addition of FITC-E2 dL5 FITC-E2 dL5orogen MG-2p, andearly 7-fold signal-thebackground ratio improvement is seen Such improved signal-to-background may improve sensitivity fluorogention of low-abundance antigen on cells.
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