Significant signal overlap was also observed between ORF38 and the late endosome marker.
A large uncertainty is also introduced by signal overlap in the 13C and 1H spectra.
For example, a frequency offset between cooperating nodes will result in signal overlap, beamforming distortion, and degraded performance [70].
APT (attached proton test) and DEPT (distortionless enhancement by polarisation transfer) spectra were acquired to enable proper resonance assignments, especially in the regions with severe signal overlap.
The results are used to design the space among enzyme-immobilized pixels and the null H+ sensor to minimize the undesired signal overlap by H+ diffusion.
This combination results in fluorescence signal overlap from the different dyes employed and also requires as many excitation sources as the number of dyes used.
In particular, for quantification of the nuclear Overhauser effect (NOE) it is highly desirable to utilize pure shift techniques where signal overlap normally prevents accurate signal integration, to enable measurement of a larger number of interatomic distances.
These properties of QDs allow multicolor QDs to be excited from one source by common fluorescent dyes without emission signal overlap and results in brighter probes comparing to conventional fluorophores.
Helios also provides improved sensitivity to detect and resolve lowerabundance targets and an expanded mass range of 135 discrete channels with virtually no signal overlap or background noise as compared to conventional techniques.
However, further analysis of the spectra is significantly complicated by the small chemical shift dispersion and signal overlap (Fig. 6).
These values are consistent with a signal overlap of TbMyo1 with each marker of approximately 50% within the population.
