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Exact(2)
Equation 1 defines the detection limit (y), which indicates whether a sample contains a certain Stx based on the average (y B) and standard deviation (SB) of the signal from a blank control (signal of samples without Stx).
We estimated the hypomethylation level of each MspI/HpaII site in the three chromosomes by determining the log ratio of the signal of the control samples to the signal of samples digested with HpaII: log(HpaII−/HpaII+).
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One can observe that the Si oxide signal of sample B is obviously larger than that of samples A and C, while almost no Ge oxide signal is measured on sample B together with the large decrease of the Ge 3d signal.
The implementation of this method starts with auto-correlation of a window of CP length samples with signal of N samples apart and moving the window sample by sample.
Here, it is noted that the (010) reflection signals of samples are obscured generally within the 2D GIWAXD images.
In addition, DNP enhanced NMR signals of samples containing TEMPO and trityl are followed as a function of the MW frequency.
Isotype controls prepared analogously were run in parallel to assess background signals of samples that showed potential artifacts in flow cytometry.
It indicates that the (010) reflection signals of samples is present indeed in the q xy direction, which can be indicated by the one-dimensional integrated curve stem from the Fig. 4a, b, c.
Manual override was used in about 1.5% of the signals that were defined by the algorithm as problematic, as well as all signals of samples which were amplified using Genome-Plex, due to their high stutter patterns.
Briefly, fluorescence signals of samples excited at 488 nm (F488) and at 458 nm (F458) were measured and the ratio (F = F488/F458) calculated.
Those probes were designed at SNP positions, where the signal intensities differed depending on the SNP genotypes of samples, which led to non-optimal copy number judgment when the signals of samples with different SNP genotypes were compared (4).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com