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Isolation of oxygen-tolerant mutants by FACS requires that a fluorescent signal develop in the presence of an active hydrogenase surviving oxygen exposure.
Even if the size decreases, but areas of high signal develop this indicates recurrence and these changes may precede symptoms by 8 12 weeks.
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A signal develops when the helium enters through the leak, and the exact location can be determined by adept manipulation of the capillary.
Initially, a focal increase in BOLD signal developed within extrastriate cortex.
The reference signal developed at the output of the algorithm is unstable due to frequent change in atmospheric condition.
Blots were washed eight times with 1× PBS with 0.05% Tween-20 and once with 1× PBS and then signal developed with SuperSignal West Pico Substrate (Pierce, United States).
The tests may signal developing hearing impairment at an early stage.
The blots were washed and signal developed with Western Lightning chemiluminescence reagent (PerkinElmer) as per manufacturer guidelines.
A fluorescent signal develops if the N- and C-terminal fragments of YFP come within 5 nm of each other.
The blots were washed and the signal developed with Western Lightning chemiluminescence reagent (PerkinElmer) according to the manufacturer's protocol.
As in a number of interferograms, the production wells inspection reveals that surface deformation signals develop likely due to extraction in a period of several months.
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