Exact(11)
The sections were processed for 5-HT immunofluorescence staining using the TSA (Tyramide Signal Amplification) method, and then for NK1 or NK3 staining with routine immunofluorescence staining.
Based on the protective performance of polydopamine nanospheres (PDANSs) for DNA against nuclease digestion and the specific recognition characteristic of aptamer, we have developed an enzymatic recycling signal amplification method for highly sensitive and selective detection of adenosine triphosphate (ATP).
In this proof of principle, the sensor was designed for the detection of mercury (II) ions (Hg2+) based on the Förster Resonance Energy Transfer (FRET), and the catalyzed hairpin assembly (CHA) technique that was used as the signal amplification method.
With any exponential signal amplification method, nonspecific amplification due to exponential artifacts presents a potential problem.
The test principle employs the Invader® chemistry, which is a signal amplification method recognizing specific nucleic acid sequences.
The protein expression was detected by the ImmunoMax/catalyzed signal amplification method with 3,3-diaminobenzidine tetrahydrochloride as a substrate [ 22].
Similar(49)
This chapter discusses the signal amplification methods in molecular diagnostics.
Together with his team, he is planning to engineer a DNA nanotechnology-based approach for highly multiplexed detection of molecules within human cells in their natural tissue and organ context using advanced signal amplification methods.
This system provides a detection limit of 160 pM, which is comparable to the existing signal amplification methods that utilized Exo III as a signal amplification nuclease.
The methodology used for the amplification of the signal has a large impact on the linear relationship between target and detected signal, with not all signal amplification methods being amenable to direct quantization.
This strategy possesses some remarkable features compared to conventional signal amplification methods: (i) A smart probe including a label-free aptamer is fabricated, for suitable hybridization without hindering the affinity of the aptamer toward its target.
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