A third group received LV-MSK-1, on only one side (Supplementary Material, Fig. S1).
Moreover, traR generally is located in an operon the expression of which can be regulated by a specific external signal (fig. 1 B, left side and supplementary fig. S1, left side, Supplementary Material online).
Shh receptor Ptch1 was predominantly expressed in neurons in the ischemic cortex and occasional Ptch1+ neurons were found on the contralateral side (Supplementary Figure S1D).
This is particularly interesting, as our methodology in which side chains with very large amplitudes of movements were excluded from the dataset would shift the distribution towards the left-hand side (Supplementary Fig. 2).
Moreover, unlike in the Group I plasmids where traR generally is a member of an operon, in the Group II plasmids examined to date traR is monocistronic (fig. 1 B, right side and supplementary fig. S1, right side, Supplementary Material online).
In the Group II plasmids the traI/trb operon again is divergently linked to repABC, but the tra locus is contiguous to the trb region with traM and traR located between these two components (fig. 1 B, right side and supplementary fig. S1, right side, Supplementary Material online).
In Group I plasmids, a locus encoding traR, traM, the two divergently oriented tra operons, and the cis-acting oriT is separated, often by more than 60 kb, from the traI/ trb genes (fig. 1 B, left side and supplementary fig. S1, left side, Supplementary Material online).
Mutation of the charged residues in the vicinity of Asp1023, including E862K (Supplementary information, Figure S5C, Scp1-M7) anD916K/E919K9K (Supplementary information, Figure S5C, Scp1-M8) had no detectable impact on Sre1 binding, suggesting that Asp1023 may demarcate the Sre1-binding surface of Scp1 on one side (Supplementary information, Figure S5A).
Analysis of cross-sectional areas of the M. tibialis anterior 4 weeks after denervation indicated the expected decrease in muscle diameter on the lesion side but G-CSF did not increase the median diameter on the lesioned or the unlesioned side (Supplementary Fig. S7).
The NPC-binding domains of these nucleoporins withstood degradation longer but when the HEZs became visible, the anchor domains appeared largely degraded too, except for those of Nup358, an FG-repeat nucleoporin located at the NPC's cytoplasmic side (Supplementary Figure S3), and Nup153.
Blood islands density and morphology following the transfection of the either C124S PTEN-GFP or G129E PTEN-GFP have not alternated in comparison to control side (supplementary material Fig. S3C,D,G,H), suggesting that the lipid phosphatase of PTEN play more principal role on regulating blood islands formation.
