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All cell lines expressed stem cell markers and side populations were detected in most (Table 1).
Further evidence that neuroblastoma is a stem cell disease is the finding of side populations in 65% of primary neuroblastoma samples [7].
Cell-surface markers and side populations (SPs) were analyzed using flow cytometry.
Cell sorting substantially depends on cell surface markers, cytokeratins, side populations and aldehyde dehydrogenase.
Side populations are due to the ability of ABCG2 to efflux a DNA dye (which ABCG2-negative cells in the main population cannot), and bone marrow side populations are enriched for marrow graft repopulating stem cells.
Knockdown of FBP1 reduced cell proliferation, clonogenicity and the ratio of side populations, as well as tumorigenesis in nude mice.
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Side population.
To investigate whether stem (progenitor) cells are found in human endometrial side population cells.
To isolate and characterize human leiomyoma stem cells by the side population (SP) method.
We demonstrated that GLI2 knockdown sensitized gastric cancer cells to 5Fu treatment, decreased ABCG2 expression, and reduced side population.
Moreover, ABCG2 is the molecular determinant of the side population that is characteristically enriched in normal and cancer stem cells.
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