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Test probes were added to apical side of tissue and sampling occurred (200 μL) every 20 min for 120 min from the basolateral side, and at 0 and 120 min from the apical side.
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Briefly, 6 µCi of 51Cr-EDTA/ml was added to the luminal side of tissues.
As the polyester type material degradation, granulation tissue infiltrated into CPU3 bulk (b2) and connected the two sides of tissue with the time passing by, which might be attributed to scaffold's high hydrophilicity, faster biodegradability, and better cytocompatibility.
Fig. 55 Digested liver tissue: The left side of the tissue shows the typical morphology of digested tissue matrix after harvesting the hepatocytes.
This finding was repeated by Mira-Pascual et al. [ 19], who performed side-by-side analyses of tissue and stool samples.
Further, our polarized model also allows us to simulate systemic dosing of drug by applying tenofovir (1 mg/ml) to the basolateral side of the tissue (lamina propria) and HIV-1 to the apical side of the tissue (epithelium).
Between the lateral walls of the CP epithelial cells are complex interdigitations particularly apparent close to the blood side of the tissue laying on a basal lamina that demarcate the inner stroma of a highly vascularized connective tissue; these interdigitations expand the surface area of the CP [ 9].
One side of the tissue strip was attached to an isometric transducer that records changes in tension.
PSE consist of elongated epithelial cells that are attached to the apical and basal side of the tissue.
The epithelial side of the tissue was oriented toward the donor compartment.
To simulate systemic dosing, 1 mg/ml of tenofovir was added to the basolateral side of the tissue explant cultures.
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