At 24 h, the seeded cells migrated to the lower side of the filter membrane.
Operators of fine filtration systems need to move fluid efficiently from one side of the filter to the other while achieving their desired amount of filtration.
The coil of the transducer adds some inductance on the electrical side of the filter.
Cells that had migrated (on the bottom side of the filter) were counted using light microscopy.
The lower side of the filter was coated with 10 mg/ml fibronectin for 2 h at 37°C.
Finally, cells that have migrated to the bottom side of the filter were photographed and counted under a microscope.
Non migrated cells were scraped from the upper side of the filter, and migrated cells on the lower side counted by light microscopy.
The ability of the cells to adhere to the filters was verified by staining the upper side of the filter for each cell line.
Cells (2.5×104/well) were seeded on the upper side of the filter and incubated in DMEM medium and 0,5% FBS.
Invading cells (on the lower side of the filter) were photographed and counted.
Cells on the upper side of the filter were removed with cotton swabs.
