We have more sell-side assignments this year with no financing role than we have assignments with financing — and we still lead the tables when you take out deals with our financing.
We repeated measurements of female association times (including switching of side-assignments of the two stimulus males) as described for the 1st part of the experiments.
2D C−H HSQC, 3D CC CO NH-TOCSY, 3D HCC CO NH-TOCSY, and 3D HCC CO NH-TOCSYtra were acquired for side cHCC CO NH-TOCSY.
The structural comparison with the recently published 3.5 Å model shows major discrepancies, both in the main chain tracing and in the side chain assignment of the two terminal domains R1 and R2.
Experiments using 2D 1H-15N and 1H-13C HSQC, 3D HNCA, CBCA CO NH, HNCACB, HNCO, HN CA CO, HBHA CO NH, HBHANH, HCCH-TOCSY, and CCH-TOCSY were performed for the backbone and side chain assignments of Rpn13C.
3D 15N-edited NOESY-HSQC, 4D 13C,15N-edited NOESY and 4D 13C,13C-edited NOESY experiments were used for side chain assignments and to identify long range intra- and interdomain [1H,1H] NOEs [30].
For V22-SH3, NMR spectra were acquired for both backbone and side chain assignments which included 15N-edited HSQC-TOCSY, HSQC-NOESY and 13C-HCCH-TOCSY as well as triple-resonance experiments [HNCACB, CBCA CO NH, HNCO].
Completeness of backbone and side-chain assignment reached 96.7 and 72.7 %, respectively.
These tools, along with chemical shift predictions from the PACSY database, greatly accelerate protein side chain assignments.
Methyl side chain assignments for several intermediates of the hDHFR major enzymatic cycle are summarized in Table S1 of the Supporting Information.
