Sentence examples for shuttle expression from inspiring English sources

The enzyme was actively expressed by the Escherichia coli Bacillus subtilis shuttle expression vector pSUGV4 in the protease-deficient strain B. subtilis WB600, and its biochemical characteristics were the same as those of the original subtilisin DFE isolated from the donor strain, i.e., its molecular weight is ∼28 kDa and it is a serine protease.

subtilis shuttle expression vectors.

glutamicum shuttle expression vector carrying a tacM promoter, resulting in the plasmid pJYW-5-gadB2.

Here we have constructed a shuttle expression vector pDXW-8 between Escherichia coli and B. flavum.

pMG-CBBM, a Rhodopseudomonas/Escherichia coli shuttle expression plasmid, was derived from the Rhodopseudomonas/E.

The PCR product was digested with BglII and PstI, and ligated into pDXW-10, a shuttle expression vector between E. coli and Corynebacteria which carries a tacM promoter (Xu et al. 2010), resulting in the plasmid pDXW-10-R4a-gadB1mut pDXW-10-R4a-gadB1mut pDXW-10-R4a-gadB1mut pDXW-10-R4a-gadB1mut

Hence, we consider the two novel L. buchneri plasmids a valuable resource for the generation of shuttle and expression vectors for LAB.

Currently, general genetic tools have been developed in Z. mobilis since the 1980s, including native plasmids, broad host-range vectors or shuttle vectors, expression system, gene transfer, promoter, and reporter gene, as reviewed in other articles [ 8, 11, 13].

This is achieved by engineering a dual expression shuttle vector, pDuLX-Luc, that replicates in E. coli and rsΔ2 and drives gene expression from the Listeria promoter (Phly) as well as the eukaryotic cytomegalovirus promoter (CMV), thereby delivering both protein and plasmid DNA to the cell cytoplasm.

Class II HDACs (4, 5, 6, 7, 9, and 10) exhibit tissue specific patterns of expression, shuttle between the nucleus and cytoplasm, and are larger than class I HDACs [ 5].

Not only will a plant related knowledge base require the object plant instead of patient, a vector in virology might describe an infectious agent while in synthetic biology it will more likely define a DNA expression shuttle.