While this treatment can prevent cell proliferation, it can also shut down transcription since intact transcripts may not be able to be generated due to DNA damage.
It is possible that their transcription may have been shut down because these transcripts were no longer required until the next blood-meal.
Reduction in the number of transcripts observed in the mutant after fertilization seems consistent with shut down in transcriptional processes resulting from incomplete fertilization or failure of embryo development.
In Drosophila spermatogenesis, it is thought that global transcription is shut down upon onset of meiosis, and stored transcripts are translated, as required, during later stages.
Moreover, in L-GMP 271 transcripts were uniquely expressed and 2566 transcripts were completely shut down (Fig 7E).
This argues against a simple model where transcription would be shut off for both genes and intronic transcripts would decrease rapidly simply because they are less stable than the mature RNAs.
Interestingly, the majority of the secretory salivary gland-expressed transcripts displayed a lower abundance after blood-feeding, suggesting that their products may have been employed for protein synthesis during feeding and eventually degraded, or their transcription had been shut down.
This transcript, ASFMR1, is up-regulated in premutation carriers and shut down in fragile X patients similar to FMR1 and FMR4.
"It wouldn't cost me anything to shut our refineries in the U.S.," Mr. Chávez said Saturday, according to a transcript.
Array-based methylation analysis and comparison with transcript expression data provided evidence that KIAA1199 expression may have been shut down in normal colon mucosa by promotor methylation.
The transcripts are also filled with people telling each other to "shut up" and keep quiet, discussions about the need to get lawyers and a person fearing they would end up in prison.
