Exact(7)
The researchers attached these entry keys to PNAs designed to shut down expression of a test gene--the galanin receptor gene, which binds a protein, galanin, that helps regulate everything from pain perception to food intake.
The initial promise of being able to transiently shut down expression of any gene soon gave way to reality, as researchers discovered that their synthetic siRNAs often targeted multiple transcripts, and often lacked the potency of more traditional drugs.
It has evolved to encompass any occasion when a minority of people try to shut down expression through inappropriate means because they dislike the speech in question.
Cells were grown in YP galactose and resuspended in YP glucose media for 2 hours, to shut down expression of GAL1-driven RPS5.
When the AHL concentration is high, induction is strong and LacIM1 levels are enough to shut down expression of the FP.
Under -/- conditions, RFP is expression is induced as before; However, observe that P lac promoter induces expression of cI to shut down expression of GFP (see Figure 7d).
Similar(53)
The majority of T cells at DN3 stage commit to the αβ-T cell lineage by further upregulating key T cell genes and shutting down expression of genes that are important for non-T cell lineages [ 78].
DNA methylation may be a factor in restricting the expression of fetal genes during liver development and in shutting down expression in hepatoma cells.
In this study, we are exploring a novel strategy to overcome endocrine resistance by shutting down expression of the ER gene itself.
Altogether, these data suggest that domains or surfaces of G9a other than the histone H3 interaction surface of its ANK domain are required for shutting down expression of SSEA-1.
A majority of the genes in these groups are expressed in the developing fetal and adult liver indicating that DNA methylation may play a role in restricting the expression of fetal liver genes and in shutting down expression in hepatoma cells.
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