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Our proposed shuffling method is illustrated in Figure 6.
Results listed above suggest that the modified genome shuffling method is effective for efficient gene transfer and therefore capable of constructing stable recombinant yeast strains with enhanced fermentation performance in a short time.
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In this Q-PCR assay, a computational DNA random shuffling method was used to design an internal amplification control (IAC) sequence, which was the same in length and G + C content to the hly amplicon.
This modified genome shuffling method was fast, straight-forward, and easy to operate.
In this study, a modified genome shuffling method was developed to improve xylose fermentation by S. cerevisiae.
Combined with proper screening strategy, the modified genome shuffling method was effective and easy to operate for the construction a recombinant strain with desired phenotypes in a short time.
These results also demonstrate that the different types of shuffled methods are indistinguishable.
These data demonstrate the threshold levels have not affect robustness and the different types of shuffled methods are indistinguishable.
Given a p-value of 0.05 in the sense of [ 42 ], we calculated that 6% of the couples of positions that are detected using the random shuffling method are due to multiplicity (i. e. FDR) for the three sets.
One potentially useful byproduct of the diploid-shuffle method is to link each ts allele to the cognate barcode assigned to the heterozygous deletion mutant corresponding to the gene of interest.
One significant drawback of this DNA-shuffling method is the low frequency of chimeric genes in the shuffled library, which may be due to the homo-duplex formation of DNA fragments derived from the same parental genes at the annealing step, the probability of which is much higher than that of hetero-duplex formation.
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