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The main claim in this paper is that Cas1 shows a clear sequence preference for nucleotides at the leader-repeat boundary but this is not true for both sites of integration.
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Other SVs did not show a clear sequence signature.
The sites show a clear sequence motif resembling the known ADAR motif (depletion of G/C in 5'; enrichment of G in 3').
Lastly, these sites show a clear sequence motif resembling the known ADAR motif: depletion of G/C in 5' (only 8% and 12% of these sites have G and C in the 5', respectively) and enrichment of G in 3' (41% of these sites have G in the 3').
However, a clear sequence bias is evident.
In core LEF 50, subunit I shows a clear fining-up sequence.
The BLAST atlas shows a clear distinction between species, as sequence identity is higher within a species than between different species for most genes (fig. 2).
Although the effect is small, it shows a clear difference between the real RNA sequences and artificial sequences that were randomized by di-nucleotide shuffling.
A phylogram of aligned aa sequences shows a clear separation into two classes (Figure 4b).
The phylogenetic tree of all 291 full-length H6 gene sequences shows a clear division between the Eurasian and North American clades (Technical Appendix Figure 1).
Notably, only Sam50 shows clear sequence homology with non-mitochondrial proteins: Omp85 in bacterial and Toc75-V in chloroplasts [ 10- 12].
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CEO of Professional Science Editing for Scientists @ prosciediting.com