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Although exogenous BNP inhibits ASMA mRNA expression in static cardiac fibroblasts (data not shown) we demonstrate here that it has little effect on ASMA protein expression.
Whilst KF-Cy3b is a monomeric sample not prone to aggregation (data not shown), we demonstrate that cell filtration is also a convenient means of removing protein aggregates from cell suspension.
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By immunofluorescence staining on spleen sections, using an antibody recognizing exclusively human vimentin and not mouse vimentin as verified by western blotting on mouse 3T3 cells (data not shown), we demonstrated that aMSC and pMSC survived in the spleen up to 8 weeks after transplantation (Fig. 5A).
For example, in preliminary experimentation with SLIDE (data not shown), we demonstrated successful SLIDE operation with droplet volumes ranging from 10 to 400 μL.
Although a similar level of p53 accumulation was also observed in hif2α morphant embryos (data not shown), we demonstrated that inhibition of p53 expression did not prevent massive apoptosis in hif2α morphant embryos.
Although no differences in locomotion were noted during initial habituation to the apparatus (results not shown), we previously demonstrated increases in adult locomotor behavior in a novel open field and on an elevated plus maze following adolescent social defeat [ 18, 19], as well as upon initial exposure to a CPP apparatus [ 20].
Adams said the swap deal showed "we have demonstrated to the bank that Jessops has a valid place in the market and a valid business plan and that there is good chance of getting a return".
From the experiments shown here, we demonstrated the importance of CH hydrogen bonding element as a decisive modulating moiety for anionic recognition.
TGF-β was not significantly altered following alveolar macrophage manipulation (data not shown), and we demonstrated elevated IL-10 levels (Fig. 2H).
Here, we further discuss why our current study provides a substantial new understanding of visual cortical correlation patterns beyond what previously has been shown: 1) We demonstrated a topography of correlation patterns that reflects the underlying, fine-scale eccentricity organization.
Using GFP reporter constructs (data not shown), we could demonstrate that in activated and transfected murine T cells a higher percentage of the T cells was GFP-positive when compared to naïve, unstimulated murine T cells.
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