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Based on these results and additional transient transfection experiments (data not shown), we constructed a cell line that carried integrated miR-122 expression vector (NIH3T3-47/miR-122) in mousembryoyo fibroblasts, which also stably express the Rheoswitch proteins.
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Section 2 shows how we constructed the data from various administrative data sources, displays descriptive graphs and presents our empirical strategy.
"The released results from the pilot exercise showed we had successfully constructed [research quality] profiles and the exercise was a success.
In order to confirm that these signals show endogenous Tmub1/HOPS, we constructed Tmub1/HOPS-RNAi plasmids having sequences corresponding to 519 537 base pair (bp), 134 152 bp, and 732 750 bp of Tmub1/HOPS.
As shown in Figure 3A, we constructed a control plasmid pLacZ, in which the expression of LacZ ORF was controlled by the GAP promoter.
As a result, we show that the ISTs we constructed are optimal in the sense that both the heights and the average path length of trees are minimized.
Because an extrachromosomal (Ex) line sometimes shows unstable mosaic expression, we constructed the chromosomally-integrated line MJCU049 {kIs48 [gst-4p::gfp, pDP MM016B] V} as a nematode biosensor.
Figure 1 shows the three networks we constructed, with edges between social pairs removed.
Two mutants we constructed showed significant enhancement in transduction efficiency in liver, especially for the S503A which exhibited rapid gene expression and maintained higher gene expression level at all the time points investigated.
As RNA interference triggered by shRNA constructs has been shown to be particularly efficient, we constructed plasmid vectors to express these interfering sequences as shRNAs under control of the human U6 promoter.
To test whether the candidate SNPs selected from SNPs-seq also showed allele-dependent enhancer activities, we constructed a plasmid library by inserting PCR-generated fragment pool (see Supplementary Data 3 for primer sequences) into STARR-seq vector.
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