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HACAT cells were treated in vitro with DNA demethylating agent 5-aza-2′-deoxycytidine (A) (1 μM for 72 h) and the histone deacetylase inhibitor trichostatin A (T2) (1 μM for 36 h) as shown in the Material and Methods.
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In the end you failed but in the process you caused horrendous injuries as shown in the materials before the court.
It is an example of a problem wherein knowledge not shown in the materials is used.
Methods for calculating the theoretical mean values of DSBs and DSBCs are shown in the Materials and Methods section.
The Z-score is calculated from the mean and variance of the Poisson-binomial distribution as shown in the Materials and Methods section and published in [35].
For each dataset, the brain functional networks were constructed and then analyzed with the same criterion shown in the Materials and Methods.
OTUD-6B antibody production, OTUD-6B recombinant protein, Otud-6b enhancer luciferase plasmids, and Otud-6b siRNA design were shown in the (Materials and Methods S1).
55 patients with preserved eGFR (>90 ml/min per 1.73 m2) were also considered as CKD patients (early stage) based on the recruitment standards shown in the Materials and Methods section.
With respect to the temperature, the determined ex vivo values can be corrected as shown in the Materials and methods section; but correction for the effect of fixation on cellular metabolism is not possible.
The average time for 1-bp DNA to open or close on the histone surface is expected to be less than 1 ms (a derivation is shown in the Materials and methods section).
We estimated growth parameters by brood year from back-calculated fork lengths weighted by the estimated number of fish alive at a specific age per number of samples shown in the 'Materials and methods'.
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CEO of Professional Science Editing for Scientists @ prosciediting.com