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Furthermore, an ACC simulation results using a stop&go scenario are shown, demonstrating a better fulfillment of the design requirements.
Snail deficiency did not impair IL-1β induced NF-κB activation in HCT116 cells (data not shown), demonstrating a specific requirement for Snail in macrophage mediated β-catenin driven transcription.
No significant increase in fluorescent signal was observed when using an FPR specific antibody on either cell type (data not shown) demonstrating a lack of FPR receptor protein in these cells.
Levels of TNFα in culture supernatants of infected macrophages were also measured and it was found that only macrophages infected with subsp. novicida exhibited significant levels of this cytokine (data not shown), demonstrating a lack of correlation of rat BMDM TNFα production and the ability of the individual F. tularensis strains to replicate intracellularly.
Furthermore, using phalloidin stainings to assay cell size, we noted that ectopic expression of Cyclin D/Cdk4 led to larger cells, which again was abolished in the delg mutants (data not shown), demonstrating a functional link between Cyclin D/Cdk4 and Delg.
In particular, the control of upper glycolysis genes by acetylation was shown, demonstrating a feedback control loop from the TCA cycle to glycolysis.
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When the pH of each mineral leachate was adjusted to pH 7.0, using 1 M sodium hydroxide, bactericidal activity was eliminated (data not shown), demonstrating an important role for low pH conditions in antibacterial activity.
a Coronal ultrasound through the atria at 30 weeks' gestation with non-visualisation of the CSP (not shown) demonstrates a supratentorial cyst (asterisk), with a differential diagnosis of porencephaly versus schizencephaly.
Chest radiograph on admission (not shown) demonstrated a 2.9 × 4.4 cm soft tissue mass in the anterior mediastinum.
Initial quantitative RT PCR experiments (results not shown) demonstrated a highly variable level of expression in prostate cancer with low levels in a range of normal tissue as follows: the thymus, thyroid, breast, colon, liver, lung and bladder.
In line with this, preliminary in vitro results (data not shown) demonstrated an apoptosis sensitization by pentoxifylline.
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