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First, the antibodies showed high specificity in ELISA by serial dilution (data not shown) and, second, by competition with the immunizing peptide in a peptide competition assay.
The phylogenetic trees obtained with the first (not shown) and second data set (Additional file 6) included the protein CrPDI-2 of C. reinhardtii in the second major clade, together with proteins of the IV and V phylogenetic groups.
As controls, we verified first that the Idefix fragment has been indeed excised after the heat shock treatment (PCR amplifications not shown), and second, that GFP expression is not due to the heat shock treatment, but indeed to Idefix excision since no GFP was detected in cells with the [Actin-Gal4/+; tgeneticTM6] genetic background after heat shock.
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Selected results from the quality assurance are shown and first lessons are drawn from the experience gained during module production.
Boxes show first and third quartiles, with the median as a thick line.
The contour plots showing first and third principle stresses also correspond with the general rotation or translation of the maxilla.
In contrast, Scn3b−/− hearts showed first and third degree heart block.
ExomeDepth (0.67) and ExCopyDepth (0.34) showed second and third highest TP/FP ratio indicating lower FP CNV counts.
This exhibition, the third show and first survey of Sobel's work, contributes to that unfolding.
The country's first popular culture, the minstrel show, and first homegrown art form, the blues, both celebrated the footloose traveler.
It's just one of those things". Moore was a secret celebrity guest star in the Broadway show and first performed on Tuesday.
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