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Transcriptome analysis with microarray revealed the achievement of dormant state showing repression of energy generation, transcription and translation machineries and induction of stress-responsive genes.
These reports are in line with our data showing repression of the miR17 92 cluster in old failing hearts.
There are in total 9088 regulator-target pairs showing activation and 13,167 regulator-target pairs showing repression in the database.
For example, FOXO3A (in our PI3K mRNA signature, one of the genes showing repression by PI3K (i.e., induction by PI3K inhibitors) can bind the ER promoter and increase ER levels, and HER-2/Akt-mediated activation and translocation of FOXO3A out of the nucleus results in a reduction of ER levels [ 53].
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Twelve miRNAs showed activation and gain of repressive epigenetic marks and six (including the chromosome 14 miRNA cluster) showed repression in combination with active chromatin (Additional file 1, Table S1).
Results: Twelve genes exhibited significant up-regulation and 27 showed repression in hearts perfused for 60 or 120 min as compared to 0 min controls.
Notably, the GH3 gene showed repression only in M21.
A recent study has shown repression of Pax6 promoter via TGF-β signaling [47].
We found that all of these miRNAs showed repression of 20 27% of luciferase activity.
The present study showed repression of 4 Htrs and induction of 11 transposon elements.
A few lines (e.g., number 66) showed repression; perhaps due to over-expression in a heterologous system.
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