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To test this hypothesis, all FACs were removed from OS Sl using an ion exchange column and the FAC-free OS Sl was applied to wounds in N. attenuata leaves, a species showing induced JA accumulation in response to both FAC and OS Sl.
Lane 2: Recombinant P. pastoris showing induced band of SAM synthetase (~ 42 kDa).
To confirm the differential expression of immune-responsive genes, four highly abundant genes in our CaEST dataset showing induced gene expression were selected for RNA blot analysis.
For all flies showing induced behavior, the temperature was immediately increased to 25° after the 2 min at low temperature, and behavior was observed for an additional 2 min. This functioned as one type of negative control, because the TRPM8 channel has been shown to be inactive at 25° (Peabody et al. 2009).
Coupled with CHIP analysis showing induced binding of PXR to the UGT1A1 gene following DEX treatment (Fig. 4C) along with previous experiments demonstrating that PXR binding to this region of the UGT1A1 gene stimulates transactivation of the promoter, 3 these findings confirm that induction of UGT1A1 is closely linked to activation of PXR during pregnancy.
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These species showed induced JA accumulations only in response to OS Sl and no induced TPI activity at all.
Figure 1b shows induced mined tree patterns, embedded patterns and non-redundant homomorphic patterns.
The cardiomyoblast H9c2 shows induced cell adhesion and cytoskeleton organization on nanodot arrays smaller than 50-nm[20] 50-nm[20]
(a) Thermal mediated cross-linking FAVE polymers with enchained fluoro-olefins show induced thermal cross-linking of internal fluoro-olefins on heating to >325 °C [23].
In control patients, neither P31-43 (Fig. 2 B) nor PTG (not shown) induced any proliferation [9].
Further animal studies have shown induced insulin resistance and T2DM in mice [ 66, 145, 153– 153].
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