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However, PI is still able to differentiate between dead and live cells by showing condensed nucleus in dead cells if examined immediately after staining.
Compared with the PBS treated group, tumor tissue sections isolated from cinnamon treated group (CE) showed enhancement of apoptotic population showing condensed chromatin at the nuclear membrane (crescent formation), dissolution of the nuclear membrane, and apoptotic nucleus surrounded by a rim of cytoplasm and plasma membrane [ 29].
Moreover, the FMD compound also caused clear changes on nuclear morphology, showing condensed nuclei in the treated cancer cells, which is typical of late-stage apoptosis.
Cell viability was determined by the MTT [3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (Calbiochem) assay as described previously. Hoechst 33342 (Sigma) was employed to stain dying cells showing condensed and fragmented DNA.
The total cell number, at least 300 cells from each experiment, was counted under DIC optics, and the number of cells showing condensed or fragmented nuclei in Hoechst staining was calculated under epifluorescence optics by a blinded observer.
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Surprisingly, PAC-1 induced chromatin condensation even in MCF7 cells after 24 h and almost 60% cells showed condensed nuclei after 48 h of treatment although it was earlier and more in MCFC3 cells.
a Control rats: normal neurons are shown condensed, regularly shaped, round nucleous (N) and nucleoulus (NL) in normal cells.
To this end, the ability to identify value streams is of central importance, since value streams show condensed financial information and are vital to all parts of a business, as described in Porter's value chain (Porter 2000).
A431 or HeLa cells incubated with H35N for 24 hr distinctly showed condensed nuclei in comparison to the untreated cells (Fig. 2).
They showed condensed, shrank and aggregated shapes.
The apoptotic cells showed condensed and fragmented DNA characteristics.
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