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As TbLRRP1-depleted cells showed detached flagellum (Fig. 5A) and were unable to divide (Fig. 3D, E), the duplication of FAZ was examined.
RPE cells appeared enlarged and projected into the subretinal space, which sometimes showed detached and rounded RPE cells containing typical melanin accumulation.
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They were cycled in 6 M KOH solution, and owing to the flexibility of the supporting nickel strips, did not show detaching.
The result was a fizzing, iridescent sugar rush of a show, detached from the spangly, shallow pre-teen dramas and lumbering faux-anime that seem to dominate children's TV.
For the international community to think the peace plan would work simply showed how detached it was from the reality in Syria.
Results showed blebs detaching from the entire cell surface (Fig. 2B C, arrowheads), including the flagellar membrane (Fig. 2D, arrowheads), after 4 hours of treatment using the IC50 concentration (15.12 µM to nelfinavir and 16.47 µM to lopinavir).
As further differentiation of PAX6+ cells in adherence was inefficient (data not shown), we detached the cells on d8 and replated them as small clumps into suspension culture (Fig. 1c).
Time-lapse video microscopy of differentiated cultures, induced to die by hormone withdrawal, shows cells detaching from the dish and the eventual collapse of the domes.
After 3 hours of HAMLET treatment, cells treated with 7 and 21 µM of HAMLET that had detached showed 70 and 59% viability, suggesting that detachment preceded death.
Too many Radio 4 comedy shows feel detached from the person hosting them: overworked scripts and elaborate setups can kill a connection with the listener.
A supplemental movie at 10 h shows LM8 cells detached from the original colony (Supplemental movie 1), but Dunn cells did not (data not shown).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com