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First, analysis of in vivo binding is necessary to understand Hox target specificity; the Hox genes encode a set of closely related DNA-binding transcription factors that exhibit clear functional specificity in vivo but show little binding selectivity in vitro (reviewed in [5]).
The intermediate-molecular-weight bands show little binding at either pH.
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The P-EIM impedance responses show clearly that the presence of Mg2+ is critical for imatinib to bind to Abl1, because imatinib shows little binding in the magnesium-free control buffer.
However, inactive pro-forms of MMPs showed little binding affinity for the microspheres indicating that the interaction was dependent on MMP activation.
When the synbody was screened on the ProtoArray, it showed little binding to AKT2, or AKT3 on the array, which share 92% and 87% sequence identity with AKT1, respectively.
However, the GST (negative control) showed little binding to NBD-cholesterol (Fig. 3, GST).
A TRTK consensus sequence at the N-terminus of the IC3(20) is apparent, although this region (IC3-N25) showed little binding to S100B.
The small amount of protein that does reach the surface of the cell shows little binding affinity for its partner, β-neurexin, suggesting possible misfolding of the protein.
KM1334 recognized FGF8b and FGF8f specifically out of the four human FGF8 isoforms, and showed little binding to other members of the FGF family.
For example, previous studies indicated that RAD51 shows little binding preference for single-strand DNA (ssDNA) over double-strand DNA (dsDNA) [ 3, 29].
Similarly, rifampin is bactericidal against S.aureus, has almost complete oral bioavailability, and shows little binding to serum proteins; however, it also has a low threshold for the development of spontaneous resistance during therapy [ 29].
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