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XL PCR products showing length differences when amplified from different treponemal strains were used for DNA sequencing using the Taq DyeDeoxy Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).
TPI intervals showing length differences after restriction analysis were sequenced (corresponding accession numbers are shown in Table S2).
Dictyoceratid intergenic regions show large length differences, resulting in mtDNA size variation of approximately 20% among the analyzed species.
Of the 937 pairwise comparisons, 83 showed significant length differences [see Additional file 4].
Of the remaining 511 ORFs, 127 ORFs differed in total length by at least 6 bp and showed a length difference in the repeat region of at least 6 bp.
Thus, we discarded all sequences showing a length difference of at least one base.
The total length of the genes combined was 247,338 bp in pepper and 195,342 bp in tomato, showing a length difference of 51,996 bp.
Moreover, 18S rRNA trees show striking branch length differences between species, accompanied by a conspicuous clustering of taxa with similar branch lengths.
They estimated that approximately 19% of the genome showed significant map length differences between reciprocal crosses.
Among the chromosomes showing significant map length differences, the average difference in map length between reciprocal crosses was 22%.
Similarly maps made only with dominant protein-coding genes from each F2 population showed the same trends in map length differences (data not shown), suggesting that they are not dependent on the number or types of markers used.
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