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Panels C, D, G, H, K, L show FRET measurements where purple pixels indicate regions of highest tension (low FRET).
Our experimental measurements show FRET can enhance the optical efficiency of a LSC by at least 24.7%.
Toward this end, we want to report here the ability of fluorescent unnatural nucleoside, triazolylphenanthrene (TPhenBDo) to show FRET interaction upon hybridization with fluorescently labeled natural nucleosides, PerU or OxoPyU or PerU, forming two stable chimeric DNA duplexes.
Fusion of fluorescent proteins to the carboxy-termini of α and β subunits show FRET efficiencies of 15% 20% corresponding to a distance of 62 Å–66 Å (Table 1).
The βPS-GFP/talinIBS2-mCherry pair did not show FRET in muscles, but showed substantial FRET in wing adhesions.
The intermediate decay time can be attributed to bigger oligomers that show FRET from the AF488 to AF647-labeled subunits.
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One is a single mother (Lisa Vidal, in a notably three-dimensional performance); another, the captain (Bonnie Bedelia, in a listless showing), frets over a wayward daughter and conducts a hotel-room affair with a younger man; still another (Nancy McKeon) is single, likes to drink and is grimly promiscuous as she looks for love.
Damage was common at both the head-neck junction (54% showing corrosion; 88% showing fretting) and at the stem-sleeve junction (88% corrosion; 65% fretting).
Previously we have shown that a FRET based sensor made up of αo and βγ2 shows FRET loss on receptor activation [14] suggesting that non-translocating βγs dissociate from some α subunits.
Table S1 shows FRET values for individual cells using the three quantitative FRET methods.
The rest of the proteins showed FRET ratio values +/− 20% of the 1b genotype (Figure 5C).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com