The device showed a mobility of about 1.5 3.2 cm2 (Vs)−1, depending on the process temperature and concentration of PVP in the metal oxide.
The BT-containing compound shows a narrower HOMO-LUMO gap, broad solid-state absorption and has been applied to organic field-effect transistors, showing a mobility of 0.022 cm2 V−1 s−1 after optimisation of devices using self-assembled monolayers.
After treatment with Endo H and PNGase F, the broad band of Zera-Xyl showed a mobility shift as well as a reduction in the number of electrophoretic bands (Figure 3, lanes 1 3).
Any fragment showing a mobility shift was directly sequenced, to identify the variant.
RNA gel-shift assay shows that only sqt1 probe shows a mobility shift with rYbx1 (black arrow) whereas probes spanning the wnt8 3′UTR (wnt8a 1-4) or vg1 3′UTR (vg1 1-3) do not show mobility shift with rYbx1.
Samples showing a mobility shift different from the normal pattern were directly sequenced (Stabvida, Investigation and Services in Biological Sciences Lda, Oeiras, Portugal), as described [ 25].
Furthermore, immunoblot analysis detecting purified CIP8 (Fig. 3B) showed a mobility shift of FLAG-tagged CIP8 to higher molecular weights due to ubiquitination, whereas the mobility of the E2 was not altered (data not shown).
Samples showing a mobility shift in the PCR SSCP analysis different from the normal pattern were directly sequenced (Stab Vida, Investigation and Services in Biological Sciences Lda, Oeiras, Portugal) as previously described (Gomes et al, 2007).
Pre-screening for mutations in exons 12, 18 and 23 of the PDGFRA gene was carried out by PCR-single-strand conformational polymorphism (PCR SSCP) followed by direct DNA sequencing of samples that showed a mobility shift in the PCR SSCP analysis, as previously described (Reis et al, 2005).
