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The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bone – calcified cartilage – fibrocartilage – soft tissue interface.
Ideally, these models should recapitulate the biological characteristics of the tumor and of the related tumor microenvironment (e.g. vasculature, immune cells) in patients.
Key requirements are that synthetic scaffolds should recapitulate the native three-dimensional (3D) hierarchical fibrillar structure, possess biomimetic surface properties and demonstrate mechanical integrity, and in some tissues, anisotropy.
A powerful novel discovery approach should recapitulate previous findings, as well as identify novel molecular abnormalities that offer advantages relative to what is known.
Valid animal models of PINK1 parkinsonism should recapitulate the motor symptoms seen in patients as well as the underlying nigrostriatal deficiencies and neuropathological findings.
If microtubules did indeed provide the underlying scaffold on which ICP0ΔRING protein accumulated in linear arrays (Fig. 5, 6), then inhibition of microtubule polymerization with nocodazole [57] should recapitulate the effect of ICP0's RING finger domain.
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This finding is significant, as Cz treatment should theoretically recapitulate the Δgcs1 phenotype, as shown above with membrane integrity experiments.
Clustering based on the similarity of MAE profiles should, therefore, recapitulate embryonic lineage.
Such a mouse should faithfully recapitulate human disease symptoms; this is particularly true for point mutations.
Thus, an ideal mouse model of SMA should faithfully recapitulate the cellular levels of SMN protein seen in patients in order to preserve the relative selectivity for motor neuron degeneration.
Musculoskeletal tissues possess extensive vasculature, which should be recapitulated in tissue-engineering approaches.
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