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Each LP and RP primer should have amplified about 1.1 kb of genomic DNA if no T-DNA insertion was made.
The RB and each RP primer should have amplified an approximately 0.6-kb band if T-DNA was inserted in the first exon.
In any case, the PCR followed by the self-ligation assay should have amplified any head-to-tail, head-to-head, or tail-to-tail sequence (figure 1e).
However, assuming a fairly constant revision rate, this should have amplified the difference measured between BHR and these designs.
Neither 5' nor 3' products were detected for the other 28 genes; the lack of expression of six of these 28 genes in leaves was confirmed using RT-PCR with primers that should have amplified an internal region of the transcript.
Similar(55)
It would have amplified small shifts in Earth's orbit that alone should have had only small climate effects.
All of this would have amplified suspicions.
(Mr. Mainieri could have amplified this feeling).
Would aid have amplified this feeling or prevented current tensions?
Larger political currents have amplified and complicated their efforts.
The city has issued permits for 29 rallies to have amplified sound equipment or block streets.
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