Sentence examples for shotgun libraries from inspiring English sources

Genomic DNA was randomly sheared by nebulization to fragments of 500 800 bp in length to construct two shotgun libraries.

Putative SSU rRNA gene sequences (25,316) were extracted from the shotgun libraries using GenomePeek58 and aligned to the SILVA v115 SSU database59.

Shotgun libraries (Nextera XT, Illumina, San Diego, CA, USA) were sequenced on the MiSeq II Platform (Illumina, San Diego, CA, USA).

This DNA was used to construct Whole Genome Shotgun Libraries (WGSL) and for 454 sequencing.

Fosmid and shotgun libraries were from DNA purified by a CTAB method [110].

This DNA was used to construct Linker Amplified Shotgun Libraries (LASL).

For Sanger sequencing, 3 Kbp and 8 Kbp shotgun libraries were constructed using debranched MDA products.

Shotgun libraries were constructed according to the protocol of Yuhki et al. [12], with modifications.

The GS-FLX shotgun libraries and pyrosequencing using the GS-FLX Titanium reagents were carried out at the Génome Québec Innovation Centre.

For this sequencing, several shotgun libraries were constructed using inserts with sizes 0.5 kbp, 1.5 3.5 kbp, and 8 14 kbp.

Emulsion PCR was carried out with DNA∶Bead ratios of 0.3∶1 for the shotgun libraries and 2.5∶1 for the paired-end libraries.