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One room monitors marine traffic around the spill site; others are dedicated to plugging the hole by several means known as Top Hat, Junk Shot, Top Kill.
One Shot TOP 10 E.coli cells (Invitrogen) were transformed with the recombinant prodomain construct.
One Shot TOP 10 chemically competent cells (Invitrogen) were transformed with cloning reactions and were grown overnight on LB agar plates containing ampicillin (100 µg/ml) for selection.
Competent host cells (One Shot TOP 10) were transformed to kanamycin resistance, and restriction analysis was used to identify correct clones for DNA sequencing.
The TOPO TA cloning kit (Invitrogen) was employed to clone the cDNA fragment in a suitable vector (PCR II-TOPO plasmid), which was then used to transform competent Escherichia coli cells (One Shot Top 10 Chemically Competent E. coli).
Genes were cloned into the pCR4®-TOPO® vector of the Invitrogen TOPO® TA Cloning kit and transformed into One Shot TOP 10 competent cells following the manufacturer's instructions (Invitrogen Corp., Carlsbad, CA).
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Isn't this chamber shot tops?
This construct was amplified in chemical competent BL21 cells (One Shot TOP-10 Chemical Competent E. coli; Life Technologies) and used to transfect BL21 DE3) E. coli.
"Like three shots, top of my back.
Shots top scorer Craig Reid finally registered the hosts' first shot on target after 83 minutes - to ironic cheers.
4. Friday eMailbag In response to "ugly hockey," with players blocking so many shots, Top 5 subscriber G.H. writes, "Blocking shots is a product of today's equipment.
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