Sentence examples for shot cells from inspiring English sources

Amplicons were cloned into the pCR®2.1-TOPO® vector (Invitrogen) and transformed into TOP10 One Shot Cells or DH5α One Shot Cells (Invitrogen) according to the manufacturers' instructions.

Plasmids were then isolated (QIAprep Spin Miniprep Kit, Qiagen) from the One Shot Cells.

Membrane-enriched protein fractions were prepared as previously described [18] except that cells were broken in a "One Shot" Cell Disrupter (Constant Systems LDT) at maximum pressure.

In studies which determined the cytosolic amino acid and peptide concentrations in soluble extracts of D. radiodurans homogenates (Figure 4B), the cells were resuspended (1∶1) in 20% trichloroacetic acid (TCA), disrupted at 40,000 lb/in2 (One Shot Cell Disrupter) and centrifuged at 12,000×g (1 h, 4°C).

Cells were disrupted in a One Shot Cell Disrupter (Constant System LDT, Daventry, UK) at a maximum pressure of 2.5 kbars.

Cells (corresponding to 50 mg dry weight (dw)) were collected by centrifugation, washed with water, disrupted with a One Shot Cell Disrupter (Constant System LDT) and freeze-dried for 72 h.

The cells were harvested by centrifugation and resuspended in lysis buffer (50 mM Tris HCl buffer, 50 mM NaCl, 1 mM EDTA, pH 8.0), then disrupted by One Shot Cell Disrupter (Constant Systems, British).

PCR products were TOPO TA cloned and transformed into One-Shot cells (Invitrogen).

For snap shots, yeast cells were fixed with 4% formaldehyde in PBS buffer for 30 min prior to observation.

Total recovered DNA was transformed into One Shot TOP10 cells (Invitrogen) and plated on a kanamycin containing agar plate.

RACE products were cloned into a pCR®2.1-Topo® vector (Life-Technologies Ltd., Invitrogen, Paisley, UK) and transformed into TOP10 One Shot® Cells (Life-Technologie).