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In general, data associated with the longest follow-up was retained; however, in instances where patient groups within the same study had variable lengths of follow up, estimates were made for all groups at the shortest time point to allow all groups equivalent time to accrue events.
Data were plotted using the RTCA software as cell index (CI) value over time, and the slope of CI between 0 and 24 h (TCCSUP) or 0 and 36 h (MB49/MB49-I MB49/MB49-I as the shortest time point showasg high enough chosen
The monolayers were then treated with 1 ml of fresh culture medium containing 5 mg/L of colistin sulfate (Sigma-Aldrich, Milan, Italy) for further 30 min, the shortest time point that resulted in the killing of all extracellular bacteria added to the monolayers.
Some regions of the protein, such as residues 99 109, were completely deuterated within the shortest time point employed (150 s at 0°C, pH7.0), while other regions, such as residues 58 62, 122 124 and 155 157, were not deuterated at all even after the longest time point (5000 s at 0°C, pH7.0).
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DRβV5-transfected MelJuSo cells were pulse labeled with 35S-methionine for 10 min. (At this short time point, the majority of labeled MHCII and Ii chains localize to the ER).
We did not choose a shorter time point for this assay as the complete turn-over of luciferase, which has a half-life of 3 h when inside cells, will take approximately 12 15 h.
At the shorter time point, the Rab7 Cmutantutant expressing cells showed diminished levels of the activated Erk1/2 in the nucleus compared to GFP expressing control cells and consequently predominant cytoplasmic distribution of activated Erk1/2 (Figure 5A).
No stenosis, thrombus formation, or inflation occurred in this short time point.
If mortality was assessed for more than one time point (ie, at 30 days and 1 year), then we used the shorter time point in our analysis.
This value was taken as the slope of the linear correlation (concentrations vs time) from short time point intervals (3 min–60 min).
Because the wound assay is performed at a short time point (6 h), effects on proliferation cannot account for this difference.
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