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Obviously, the peak represented by the long size class (24 27 nt) was much higher than that for the shorter size class (21 23 nt), suggesting more abundant for the longer-sized small RNAs in the ovary.
Under the same amount of ingredient for crystals to grow, the more seeds concentration indicates the more nucleation sites and the shorter size of the nanocrystals [31].
Therefore, the cDNA amplification product is expected to have a shorter size than the amplification product from genomic DNA.
As a result, RNA of shorter size would be isolated preferentially over longer RNA, which would compromise the subsequent gene expression analysis.
The selection of putative ancient insertions was prioritized as follows: first, those copies 7% 10% divergent relative to the consensus were recruited; among these divergent insertions, and to facilitate the PCR process, those displaying shorter size (i.e., 150 300 bp), and without indels and/or unsequenced gaps, were selected for the PCR optimization process.
β-actin 3'/5' ratio are notably higher in the WT-Ovation One-Direct system compared to those obtained by WT-Ovation FFPE, perhaps due to the shorter size distribution of cDNAs, suggesting increased 3' over-bias and under-representation of 5' targets (Table 2).
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RNA circles of this or shorter sizes could be amplified via RCA without the need for separate strand-displacement activity of RNA polymerase ribozymes.
In short, size small is the word on the street.
Its short size also meant it fell over less often, reducing spoilage.
Inside, is a pair of shorts (size: LARGE) made of white lace.
Eto'o has dropped a short size since leaving the comforts of Dagestan.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com