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We report an efficient approach to analyze short sequence data for discrimination between species.
However, this is less likely with short sequence data because the translations from non-coding frames are usually too short (due to random occurrence of stop codons) to rank as pORFs using our selected cut-off threshold.
For the analysis of NGS data, it is necessary to assemble these millions of short sequence data to extract sequence features of DNA samples, such as detection of single nucleotide polymorphisms (SNPs) and de novo sequencing [3].
We used these data to address the following questions: First, to what extent can this type of short sequence data be assembled de novo to full length transcripts?
In order to investigate this result, MAF obtained from short sequence data and from genotyping were simulated and correlations were calculated.
Phylogenetic relationships among mtDNA haplotypes of the ancient and contemporary grey wolves was performed using network-based methods only, because of the very short sequence data.
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However, another C.multidentata siphosome sample produced assemblies with large numbers of relatively short sequences (data unpublished).
Taking a similar approach, Serizawa et al identified the same gene using short read sequence data generated by the Illumina Genome Analyzer II sequencer [21].
While suggesting a possible fire, the relatively short sequence of data gives no insight into pilot efforts to control the aircraft, nor does it show whether it fell in one piece or disintegrated in mid-air, two aviation safety experts said.
A novel maximum-likelihood framework, adapted to transcriptome-based high-throughput short sequence read data, was here introduced for SNP and genotype calling.
breseq uses Bowtie2 to map short sequence read data to the reference genome because it performs gapped alignment, finds local matches in the read, and allows exhaustive reporting of all alignments between a read and the reference sequence [ 30].
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