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Gab posted its initial response to the shooting on Medium, but the publishing site has also now suspended the company's account.
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Through propagation of the shoots on a medium containing the selection marker, only one line (Atgai-26) was confirmed as transgenic by Southern blot hybridization (Additional file 4).
Meristematic bulks developed after successive transfers of shoots on IM medium with benzyladenine concentrations increasing up to 13.2 μM.
However, additional selection of kanamycin resistant shoots on rooting medium with 100 mg/l kanamycin yielded 95.6% transgenic plants [ 42].
In vitro shoots were inoculated on medium with IBA for 1, 2, 3, and 4 weeks and then transferred to medium without IBA for remaining 6, 5,4 and 3 weeks, respectively.
Buds and young shoots grew well on medium with 1 3 mg/l BA and 0.1-0.2 0.1-0.2A.
Configuration of the 3D mode is a pain, apparently, and the impaired light-gathering ability of the 3D add-on makes shooting in medium or low light difficult.
These number of regenerated shoots per explant was increased along with each regeneration cycles and on 3rd regeneration cycle, maximum 27.6 ± 0.53 number of shoots per explant are produced on medium containing 2.5 mg/L BAP after 8 weeks of culture (Figure 1A), whereas maximum 20.3 ± 0.33 shoots produced on 4.0 mg/L KIN containing medium after same duration.
Shoots were induced by culturing explants on shoot induction medium (SIM, MS containing 0.2 μM TDZ) (NOR-AM Chemical Co., Wilmington, DE).
On medium containing 0.5 mM urea, wild type shoots developed slightly better than dur3 shoots, as previously described by Kojima et al. [ 18].
Infected explants were placed on shoot induction medium containing either 5 mg L−1 hygromycin or 75 mg L−1 kanamycin for selection.
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