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Kanamycin at 50 mg/l was chosen for shoot selection of both cultivars because it significantly (P < 0.05) inhibited shoot growth and this non-lethal concentration may allow recovery of transgenic plants having low expression of a neomycin phosphotransferase II (NPTII).
To determine the optimal kanamycin concentration for shoot selection, we used MBCE approx. 0.5 cm diam isolated from 2-7 moldh old cultures that had been subcultured every month on IM2, and placed them on fresh IM2 with 0, 20, 35 and 50 mg/l kanamycin for 6 to 7 weeks.
For shoot selection, the percentage of multiple bud cluster explants (MBCE) producing new growth was significantly (P < 0.05) lower when using kanamycin at 50 mg/l than at 0, 20 or 35 mg/l (18.2% vs. 100%, 91.5% and 86.4% for Accent Red and 15.7% vs. 100%, 80.6% and 83.3% for Salmon Picote.
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He may well build on the 33.7 percent 3-point-shooting mark he posted last season, but without a substantial change in his shooting selection, Iguodala is doomed to below-average marksmanship.
"It's not shot selection," Kidd said.
As with his movement, Monfils' shot selection was characteristically unpredictable.
Djokovic earns the second service break with great returning and shot selection.
Wilkens said Crawford's most pressing areas of improvement were shot selection and his commitment to defense.
"They were shot selection and building up his safety game," Fung said.
Rivers, 22, will take part in the Small Bore Rifle category and is one of 14 competitors named in the first wave of shooting selections.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com