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Of three heat-specific gene modules, M9 and M17 were specifically identified in shoot samples, while M11 was identified in both root and shoot samples.
Root and shoot samples for metabolite analysis were harvest at 33 days after seeding.
In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.
Root and shoot samples were collected and washed thoroughly, and dried at 70°C.
Similarly, ME9 and ME17 were heat-specific Eigen genes only in shoot samples.
Spinach shoot samples were digested and nutrient contents in the shoots were determined.
The shoot samples (50 mg) were excised and immediately used for enzyme extraction.
The leaf and shoot samples were freeze-dried and weighed individually.
Three Eigen genes (ME5, ME7, and ME15) were expressed more in shoot samples, but the other two Eigen genes (ME13 and ME18) had similar patterns of expression in both root and shoot samples.
Under the salt stress, genes in the M4 module were specifically disturbed in both root and shoot samples.
During grinding of dried maize shoot samples, some contamination of non-labelled samples with 15N-labelled ones occurred.
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