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0.2 g of each root and shoot sample were digested with a mixture of 5 mL of HNO3 + 1 mL of HClO4.
Subsequently, all the shoot and root samples were individually dried in an oven at 65°C for 48 h, and the dry weight (DW) of each root or shoot sample was measured (n = 20).
Subsequently, the shoot and root samples were individually dried in an oven at 65°C for 48 h, and dry weight of each root or shoot sample was measured.
At this point, the fourth leaf was taken as the shoot sample.
The two-leaf-stage shoot sample included shoot apices and all leaves.
Secondly, a similar, albeit opposite, developmental shift effect was observed in the shoot sample of cytokinin-deficient plants, which is located more closely to the root coordinates than any other shoot sample.
Similar(52)
Of three heat-specific gene modules, M9 and M17 were specifically identified in shoot samples, while M11 was identified in both root and shoot samples.
Root and shoot samples for metabolite analysis were harvest at 33 days after seeding.
In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.
Root and shoot samples were collected and washed thoroughly, and dried at 70°C.
After 5 days of nutrient deficiency treatments, shoot samples were collected and total RNA was extracted from each sample.
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