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Any callus with no regenerated shoots were transferred to shoot regeneration medium III for further regeneration.
The regenerated shoots were sub-cultured on shoot regeneration medium II [ 39].
The regenerated shoots were sub-cultured on shoot regeneration medium II containing 4 mg/L hygromycin.
During this period, any shoots regenerated from callus (about 35%to40%0%) would be transferred to shoot regeneration medium II.
(b) Shoot regeneration frequency.
Shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars.
Carbon sources, phytohormones, and genotypes are well known to affect shoot regeneration in cultured cells.
Data were recorded for per cent callus induction, shoot regeneration, GUS expression and ploidy analysis.
It is suitable used to clarify the possible mechanism with respect to shoot regeneration in rice.
Thus, phytohormone signals may affect sucrose metabolism to trigger callus initiation and further de novo shoot regeneration in rice culture.
However, the effect of these sucrose metabolism-related genes on callus formation and shoot regeneration in rice are still unknown.
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