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Our EST analysis showed that 16 LMW-HSP genes were expressed in the heat stressed shoot library, which is significantly different from their expression in other libraries (Table 3).
The RI shoot library did not have any of the above transcripts in this given transcriptome set.
Only the SI shoot library had pathogen responsive ESTs (a chitinase-like protein, a nonspecific lipid-transfer protein precursor (LTP) and an F-box/LRR-repeat protein-20).
This EST had sequence similarity to mitogen-activated protein kinase kinase (MAPKK) that was cloned from the control RC shoot library of the same genotype.
For example, 23 TCs coding for different classes of heat-shock proteins (cluster F) were found in the heat-stressed shoot library.
Expression of these genes was also present in eight of the nine cDNA libraries, but not in field stressed shoot library (data not shown).
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A drought-specific gene (TC1995), which was highly expressed only in drought-stressed root and shoot libraries (928 ESTs – cluster H) showed no similarity to sequences in nr.
Interestingly, stem libraries showed a higher percentage of the signal transduction and defense-related response ESTs than the leaf and shoot libraries (Table 2).
Figure 2 shows a heat map visualisation of a digital Northern – based on EST frequencies in different libraries (Sterky et al. 2004) – of the OPLS-generated leaf gene list and shows that the greatest prevalence of genes was found in the shoot meristem, young leaf and apical shoot libraries.
To this end, we used the PopGenIE [ 52] CatFisher tool to identify transcription factor gene models that were over-represented in the young leaf, apical shoot and shoot meristem libraries of PopulusDB [ 53] (Table 1a).
(PDF 75 KB) 12284_2012_67_MOESM3_ESM.pdf Additional file 3: Figure S3: Transcript abundance of rice MAPKKs in Magnaporthe treated Nipponbare shoots libraries from MPSS database.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com