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The chaperonic heat shock transcripts and proteins were significantly up-regulated under combined stress compared with either heat or water deficit.
In addition, only upon heat shock, transcripts in both orientations have been observed, however, with sense transcripts being more prominent.
The environmentally induced heat shock transcripts and heat-stress related transcripts enricheat-stress relatedsplayed transcripts fold changenrichedat-stressed aninals relative to controls at Tc,Max (Table 2).
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During diapause, and in the absence of a general stress response however, the two heat-shock transcripts, Hsp23 and Hsp70, are up-regulated in females until exposed to normal environmental conditions.
We also observed that Sb03g027330 gene was highly abundant in both genotypes under stress and Sb03g028470, another heat shock transcription factor transcript was relatively abundant in the susceptible genotype only under stress (Fig. 4).
The induction of Hsp transcripts is mediated by the heat shock transcription factor (HSF) [52].
It is interesting to note that in our present study on mature soybean pollen transcriptome, there is significant up-regulation of transcripts encoding heat shock proteins as well as heat shock transcription factors HSFB2A and HSFA5 (Table 7; Figure 3).
We found two heat shock protein transcripts (BTT23758_2, BTT37030_1) and one other (BTT17701_1) that were downregulated and upregulated respectively.
We believe this would be sufficient to allow cross-hybridization with different heat shock protein transcripts from glucose-grown fungal cDNA and yield a positive signal in microarray and northern hybridizations.
Among them, dozens of heat shock proteins, transcripts responding to drought and osmotic adjustment (Ghi.4632.1.A1_at, Ghi.1478.1.S1_s_at, GraAffx.33059.2.S1_s_at, GhiAffx.52815.1.S1_at, and Ghi.766.1.S1_at), and flood (Gra.840.1.A1_at and GarAffx.29310.2.S1_s_at) were differentially regulated.
We sought to examine the extent to which the effect of heat shock on transcript abundance occurs via the up-regulation of transcriptional activity.
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