The thermal shock behaviour of (Ti,Al N-coated WC/Co-hard meTi,Al N-coatedtigated using the laser thermal shock method.
Service performance of the PEO + fluorocarbon (FC) paint treated by the improved SANP technique was estimated by thermal shock method (TSM) and neutral salt spray test (NSST).
MnO2 was homogenously doped into anodic nanotubular TiO2 by a potential shock method, in which a high potential was imposed on the anodic nanotubes immediately after anodization process.
The enzyme extract in periplasm and cytoplasm was obtained by osmotic shock method [16].
Then the plasmid was introduced into Escherichia coli strain DH5α with heat shock method (Sambrook et al. 1989).
In this kit, the temperature shock method was used to break open the cellulosic cell wall to obtain DNA.
It appeared that electroporation and the pressurized solvent extraction method for lipid extraction had the highest efficiency compared to other methods such as organic solvent, bead beating, and osmotic shock method (Kumar et al. 2015).
Transformation of E. coli strain BL21-DE3 (Novagenotypeotype: [95 F- ompT hsdSB (rB-mB) gal dcmrne131 (DE3)]) with pET-28_2,5-DKCMO pET-28_2,5-DKCMO pET-28_2,5-DKCMO method described by Chung et al. (1989).
However, the plasmid containing the dhaD gene was extracted from E. coli JM109, purified and transferred to the competent cell of E. coli BL21 DE3 pLysS using calcium chloride heat shock method (Ausubel et al. 1987).
The amplified product was ligated into cloning vector pJET1.2/blunt at the PCR product site and transferred into E. coli JM109 using calcium chloride heat shock method (Ausubel et al. 1987), resulting in a recombinant plasmid designated as pJET1.2/blunt-dhaD.
